Alteration of the ATG start codon of the A* protein of bacteriophage ϕX174 into an ATT codon yields a viable phage indicating that A* protein is not essential for ϕX174 reproduction

  title={Alteration of the ATG start codon of the A* protein of bacteriophage ϕX174 into an ATT codon yields a viable phage indicating that A* protein is not essential for ϕX174 reproduction},
  author={P. D. Baas and H Liewerink and H.A.A.M. van Teeffelen and A.D.M. van Mansfeld and Jaques H. van Boom and H. S. Jansz},
  journal={FEBS Letters},

Molecular dissection of the replication system of plasmid pIGRK encoding two in-frame Rep proteins with antagonistic functions

Regulation of the initiation of pIGRK replication is a complex process in which a major role is played by two in-frame proteins with antagonistic functions, the first description of such proteins in a plasmid of the pHW126 family.



Sequence of a 1.26‐kb DNA fragment containing the structural gene for E.coli initiation factor IF3: presence of an AUU initiator codon.

The nucleotide sequence of a 1.26‐kb pair DNA fragment containing the structural gene for Escherichia coli initiation factor IF3 has been determined and the presence of AUU as the translational initiator codon is determined.

Gene K of bacteriophage phi X174 codes for a protein which affects the burst size of phage production

The gene K mutant has an identical latent period, but a more reduced burst size than that of the wild-type phi X174, suggesting that the gene K protein, although not essential, has a role in increasing infectivity by increasing the burst size three- to sixfold.

Expression of the cloned bacteriophage phi X174 A* gene in Escherichia coli inhibits DNA replication and cell division

It appears that the A* protein has as one function the inhibition of cell division and DNA replication but not transcription or protein synthesis during phage infection.

Pleiotropic effects of mutants in gene A of bacteriophage phi chi 174

Two groups of mutants in gene A failed to terminate cellular DNA synthesis and were unable to efficiently inactivate the colony-forming ability of the host, suggesting additional functions of gene A.

The A * protein of φX174 is an inhibitor of DNA replication

It is proposed that this inhibitory activity is responsible in vivo for the shut off of E. coli chromosome replication during phi X174 infection, and has a role in the transition from semiconservative RF DNA replication to single-stranded DNA synthesis in the life cycle of phiX174.

Effect of SSB protein on cleavage of single-stranded DNA by phi X gene A protein and A* protein.

Evidence is presented that the second product of gene A, the A* protein, cleaves phi X viral DNA at the additional cleavage site in the presence of SSB, not only in vitro but also in vivo.

The nuclease specificity of the bacteriophage phi X174 A* protein.

It is concluded that different parts within the DNA recognition domain of the A* protein are functional in the recognition of the origin sequence in single-stranded DNA.

Temperature-sensitive mutation in the initiation codon of the rIIB gene of bacteriophage T4.

The sequence of a ribosome-protected region of T4 rIIB mRNA labeled in vivo is determined and a six-base sequence which is complementary to the 3" end of the 16S rRNA is identified.