Alteration of substrate specificity by mutations at the His61 position in predicted transmembrane domain 1 of human MDR1/P-glycoprotein.

@article{Taguchi1997AlterationOS,
  title={Alteration of substrate specificity by mutations at the His61 position in predicted transmembrane domain 1 of human MDR1/P-glycoprotein.},
  author={Yuya Taguchi and Katsuhito Kino and Mikio Morishima and Tohru Komano and Susan E. Kane and Kazunori Ueda},
  journal={Biochemistry},
  year={1997},
  volume={36 29},
  pages={8883-9}
}
In CFTR, a member of the ABC superfamily and a chloride channel, amino acid substitutions in its transmembrane domains 1 and 6 (TM1, TM6) have been reported to modulate the anion selectivity or ion conductance of the ion channel. In P-glycoprotein, no amino acid substitution in TM1, but some in TM6, have been reported to modify the substrate specificity of this protein. In this work, we demonstrated the involvement of His61, which is in the middle of the predicted TM1, in the function of P… CONTINUE READING