Alteration of immunoreactivity by hydrated autoclaving, microwave treatment, and simple heating of paraffin-embedded tissue sections.

@article{Igarashi1994AlterationOI,
  title={Alteration of immunoreactivity by hydrated autoclaving, microwave treatment, and simple heating of paraffin-embedded tissue sections.},
  author={Hiroshi Igarashi and Haruhiko Sugimura and Keiichi Maruyama and Yasuhiko Kitayama and Ichiro Ohta and Masuko Suzuki and Makoto Tanaka and Yoh Dobashi and Isamu Kino},
  journal={APMIS : acta pathologica, microbiologica, et immunologica Scandinavica},
  year={1994},
  volume={102 4},
  pages={295-307}
}
The effects of treatment in a hydrated autoclave (121 degrees C, 2 atm for 20 min), microwave oven (in water), and simple heating (60 degrees C overnight in distilled water or 90 degrees C for 10 min in ZnSO4) on the stainability of 56 antigens by commercially available antibodies in formalin-fixed paraffin-embedded tissue sections were evaluated. The… CONTINUE READING