Alteration of enzyme specificity and catalysis by protein engineering.

  title={Alteration of enzyme specificity and catalysis by protein engineering.},
  author={Helen M. Wilks and Jennifer Holbrook},
  journal={Current opinion in biotechnology},
  volume={2 4},
New substrate specificities can be introduced into existing enzymes for the purpose of making them more suitable for the chemoenzymic synthesis of single compound drugs and other chiral compounds. The most productive route used in the past year has involved the utilization of the catalytic and substrate-binding properties from homologous enzymes found in nature, one example being the broadening of the substrate specificity of yeast alcohol dehydrogenase. Other highlights include the creation of… CONTINUE READING