Alr2954 of Anabaena sp. PCC 7120 with ADP-ribose pyrophosphatase activity bestows abiotic stress tolerance in Escherichia coli

@article{Singh2016Alr2954OA,
  title={Alr2954 of Anabaena sp. PCC 7120 with ADP-ribose pyrophosphatase activity bestows abiotic stress tolerance in Escherichia coli},
  author={Parvati Singh and Alok Kumar Shrivastava and Shilpi Singh and Ruchi Rai and Antra Chatterjee and Lal Chand Rai},
  journal={Functional & Integrative Genomics},
  year={2016},
  volume={17},
  pages={39-52}
}
In silico derived properties on experimental validation revealed that hypothetical protein Alr2954 of Anabaena sp. PCC7120 is ADP-ribose pyrophosphatase, which belongs to nudix hydrolase superfamily. Presence of ADP-ribose binding site was attested by ADP-ribose pyrophosphatase activity (K m 44.71 ± 8.043 mM, V max 7.128 ± 0.417 μmol min−1 mg protein−1, and K cat/K m 9.438 × 104 μM−1 min−1). Besides ADP-ribose, the enzyme efficiently hydrolyzed various nucleoside phosphatases such as 8-oxo-dGDP… CONTINUE READING
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