Alphavirus cDNA-based expression vectors: effects of RNA transcription and nuclear export.

@article{Boorsma2003AlphavirusCE,
  title={Alphavirus cDNA-based expression vectors: effects of RNA transcription and nuclear export.},
  author={Marco Boorsma and Philippe Saudan and Holger Pfruender and J. E. Bailey and Sondra Schlesinger and Wolfgang A. Renner and Marlin F Bachmann},
  journal={Biotechnology and bioengineering},
  year={2003},
  volume={81 5},
  pages={553-62}
}
The construction of layered DNA-RNA replicons has facilitated and expanded the use of alphavirus vectors to vaccine development, construction of packaging cell lines and long-term heterologous gene expression. In these vector systems, the alphavirus replicon is under the control of a strong RNA polymerase II promoter and replicon RNA is transcribed from DNA before transport to the cytoplasm. Efficient RNA amplification catalyzed by the viral replicase results in high levels of mRNA and the… CONTINUE READING

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