Alpha-synuclein oligomers and fibrils originate in two distinct conformer pools: a small angle X-ray scattering and ensemble optimisation modelling study.

@article{Curtain2015AlphasynucleinOA,
  title={Alpha-synuclein oligomers and fibrils originate in two distinct conformer pools: a small angle X-ray scattering and ensemble optimisation modelling study.},
  author={Cyril C. Curtain and Nigel M Kirby and Haydyn D T Mertens and Kevin Jeffrey Barnham and Robert B Knott and Colin L. Masters and Roberto Cappai and Agata Rekas and Vijaya B Kenche and Timothy Ryan},
  journal={Molecular bioSystems},
  year={2015},
  volume={11 1},
  pages={190-6}
}
The 140 residue intrinsically disordered protein α-synuclein (α-syn) self-associates to form fibrils that are the major constituent of the Lewy body intracellular protein inclusions, and neurotoxic oligomers. Both of these macromolecular structures are associated with a number of neurodegenerative diseases, including Parkinson's disease and dementia with Lewy bodies. Using ensemble optimisation modelling (EOM) and small angle X-ray scattering (SAXS) on a size-exclusion column equipped beamline… CONTINUE READING
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Fitted scattering profile of A30P mutant α-syn + Cu 2+ ; B, Kratky plot; C, D max distribution

S5 (II) A

Fitted scattering profile of A30P mutant α-syn; B, Kratky plot; C, D max distribution

S4 (II) A

Fitted scattering profile of A53T mutant α-syn; B, Kratky plot; C, D max distribution; D, SE column elution profile; E, Guinier plot. Figures S5

S4 (IV) A

Fitted scattering profile of A56T mutant α-syn + Cu 2+ ; B, Kratky plot; C, D max distribution

S5(III) A

Fitted scattering profile of E46K mutant α-syn; B, Kratky plot; C, D max distribution

S4 (III) A

Fitted scattering profile of M1A/M5A/M116A/M127A substituted α-syn + Cu 2+ ; B, Kratky plot; C, D max distribution

S6 (II) A

Fitted scattering profile of wild-type α-syn + anti-fibril agent VK7; B, Kratky plot; C, D max distribution

S6 (III) A

S5 (I) A, Fitted scattering profile of wild type α-syn + Cu 2+ ; B, Kratky plot; C, D max distribution

S5 (I) A, Fitted scattering profile of wild type α-syn + Cu 2+ ; B, Kratky plot; C, D max distribution

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