Alpha-helix formation in a photoswitchable peptide tracked from picoseconds to microseconds by time-resolved IR spectroscopy.

@article{Bredenbeck2005AlphahelixFI,
  title={Alpha-helix formation in a photoswitchable peptide tracked from picoseconds to microseconds by time-resolved IR spectroscopy.},
  author={Jens Bredenbeck and Jan Helbing and Janet R Kumita and G. Andrew Woolley and Peter Hamm},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2005},
  volume={102 7},
  pages={2379-84}
}
Photo-triggered alpha-helix formation of a 16-residue peptide featuring a built-in conformational photoswitch is monitored by time-resolved IR spectroscopy. An experimental approach with 2-ps time resolution and a scanning range up to 30 micros is used to cover all time scales of the peptide dynamics. Experiments are carried out at different temperatures between 281 and 322 K. We observe single-exponential kinetics of the amide I' band at 322 K on a time scale comparable to a recent temperature… CONTINUE READING