Alpha helix content of G protein alpha subunit is decreased upon activation by receptor mimetics.

@article{Tanaka1998AlphaHC,
  title={Alpha helix content of G protein alpha subunit is decreased upon activation by receptor mimetics.},
  author={Takashi Tanaka and Toshiyuki Kohno and S. K. Kinoshita and Hidefumi Mukai and Hiroshi Itoh and Madoka Ohya and Teruo Miyazawa and Tsutomu Higashijima and Kaori Wakamatsu},
  journal={The Journal of biological chemistry},
  year={1998},
  volume={273 6},
  pages={3247-52}
}
To elucidate the mechanism whereby liganded receptor molecules enhance nucleotide exchange of GTP-binding regulatory proteins (G proteins), changes in the secondary structure of the recombinant Gi1 alpha subunit (Gi1alpha) upon binding with receptor mimetics, compound 48/80 and mastoparan, were analyzed by circular dichroism spectroscopy. Compound 48/80 enhanced the initial rate of GTPgammaS binding to soluble Gi1alpha 2.6-fold with an EC50 of 30 microg/ml. With the same EC50, the mimetic… CONTINUE READING

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