Alpha-glucan synthesis on a protein primer. A reconstituted system for the formation of protein-bound alpha-glucan.

@article{Moreno1987AlphaglucanSO,
  title={Alpha-glucan synthesis on a protein primer. A reconstituted system for the formation of protein-bound alpha-glucan.},
  author={Silvia Moreno and Carlos E. Cardini and Juana S. Tandecarz},
  journal={European journal of biochemistry},
  year={1987},
  volume={162 3},
  pages={609-14}
}
Reconstitution experiments with the DEAE-cellulose-treated enzymes, engaged in a two-step mechanism of synthesis of alpha-glucan bound to protein, are performed. Urea/sodium dodecyl sulfate/polyacrylamide gel electrophoretic analysis of the radioactive products synthesized by the reconstituted system shows highly glucosylated, labeled bands, whose apparent… CONTINUE READING