Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae.

@article{Brake1984AlphafactordirectedSA,
  title={Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae.},
  author={Anthony J. Brake and James P. Merryweather and Daniel G. Coit and Ulrike A Heberlein and Frank R. Masiarz and G. T. Mullenbach and M. S. Urdea and Pablo Valenzuela and Philip J. Barr},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1984},
  volume={81 15},
  pages={4642-6}
}
Saccharomyces cerevisiae cells were transformed with plasmids containing hybrid genes in which the sequence encoding mature human epidermal growth factor was joined to sequences encoding the leader region (preprosegment) of the precursor of the yeast mating pheromone alpha-factor. These cells accurately process the hybrid protein and efficiently secrete authentic biologically active human epidermal growth factor into the medium. 

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