Alpha complementation of LacZ in mammalian cells.

  title={Alpha complementation of LacZ in mammalian cells.},
  author={Peter Moosmann and Sandro Rusconi},
  journal={Nucleic acids research},
  volume={24 6},
The bacterial lactose converting enzyme beta-galactosidase (β-gal) and its gene (LacZ) have been studied for many years ( 1 and references therein) and are among the most utilized tools in molecular biology. The LacZ product, a polypeptide of 1029 amino acids, gives rise to the functional enzyme after tetramerization (2 and references therein) and is easily detected by chromogenic substrates either in cell lysates or directly on fixed cells in situ (3 and references therein). The… CONTINUE READING


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