Alpha-Helical Fragaceatoxin C Nanopore Engineered for Double-Stranded and Single-Stranded Nucleic Acid Analysis.

Abstract

Nanopores are used in single-molecule DNA analysis and sequencing. Herein, we show that Fragaceatoxin C (FraC), an α-helical pore-forming toxin from an actinoporin protein family, can be reconstituted in sphingomyelin-free standard planar lipid bilayers. We engineered FraC for DNA analysis and show that the funnel-shaped geometry allows tight wrapping… (More)
DOI: 10.1002/anie.201606742

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Cite this paper

@article{Wloka2016AlphaHelicalFC, title={Alpha-Helical Fragaceatoxin C Nanopore Engineered for Double-Stranded and Single-Stranded Nucleic Acid Analysis.}, author={Carsten Wloka and Natalie Lisa Mutter and Misha Soskine and Giovanni Maglia}, journal={Angewandte Chemie}, year={2016}, volume={55 40}, pages={12494-8} }