Alpha-Based Multiplexed Assay for Identifying SH2 Domain Antagonists.


Constitutive activation of STAT3/5b frequently occurs in various human malignancies. STAT3/5b activation involves dimerization via intermolecular pTyr-SH2 binding; therefore, antagonizing this interaction is a feasible approach to inhibit STAT3/5b activation for cancer therapy. We have developed a multiplexed assay to assess STAT3- and STAT5b-SH2 binding in… (More)
DOI: 10.1007/978-1-4939-6762-9_19