Allosteric effects of dexamethasone and RU486 on glucocorticoid receptor-DNA interactions.

@article{Pandit2002AllostericEO,
  title={Allosteric effects of dexamethasone and RU486 on glucocorticoid receptor-DNA interactions.},
  author={Shilpa Pandit and Wayne Geissler and Georgianna Harris and Ayesha Sitlani},
  journal={The Journal of biological chemistry},
  year={2002},
  volume={277 2},
  pages={1538-43}
}
The glucocorticoid receptor (GR) is a DNA-binding protein that can regulate the transcription of a large number of genes in a ligand-dependent fashion. Although much progress has been made on the mechanism of transcriptional regulation by GR, a potential allosteric effect of GR-binding ligands on specific GR-DNA interactions is controversial. In this study, gel-shift methods are used to measure the effects of a classical agonist dexamethasone and a prototypical antagonist RU486 on the in vitro… CONTINUE READING