Allophycocyanin 1 as a near-infrared fluorescent tracer: isolation, characterization, chemical modification, and use in a homogeneous fluorescence resonance energy transfer system.

@article{Trinquet2001Allophycocyanin1A,
  title={Allophycocyanin 1 as a near-infrared fluorescent tracer: isolation, characterization, chemical modification, and use in a homogeneous fluorescence resonance energy transfer system.},
  author={Eric Trinquet and Fabrice Maurin and Marc Pr{\'e}audat and G{\'e}rard Mathis},
  journal={Analytical biochemistry},
  year={2001},
  volume={296 2},
  pages={232-44}
}
Allophycocyanin 1 (APC1), isolated from Mastigocladus laminosus, retains the same (alpha-beta)(3) trimeric structure as allophycocyanin (APC), but incorporates a peptide linker in its core leading to a 28% increase in its fluorescence quantum yield compared to APC. Moreover, APC1 exhibits an unexpectedly good stability at very low concentrations, at extreme… CONTINUE READING