Allogeneic transplantation of normal epidermal cells and squamous cell carcinomas in SENCAR mice.

Abstract

A method used in our laboratory for the transplantation of single cell suspensions of epidermis and skin carcinomas is described. Silicone chambers were placed into a subcutaneous granulation tissue formed by the implantation of a glass disk. The skin was closed on top of the chamber by using Michael autoclips. Cells were injected 24 to 48 hr later through the skin using a syringe and needle. The neoformation of epithelia with adnexa was observed when newborn epidermal cells were injected. When a fresh cell suspension of squamous cell carcinoma was used, a typical differentiated carcinoma was formed within 2 to 4 weeks. However, after 4 weeks, some of the grafts showed mild signs of rejection. The technique described is a useful system to transplant squamous cell carcinomas and can also be used as a rapid assay for malignancy.

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Cite this paper

@article{Conti1986AllogeneicTO, title={Allogeneic transplantation of normal epidermal cells and squamous cell carcinomas in SENCAR mice.}, author={Claudio J . Conti and Jochen Walter Ulrich Fries and C. Marcelo Aldaz and Andr{\'e}s J. P. Klein-Szanto and Thomas J . Slaga}, journal={Environmental Health Perspectives}, year={1986}, volume={68}, pages={125 - 129} }