Allogeneic hemopoietic stem cell transplantation using mouse spleen cells fractionated by lectins: in vitro study of cell fractions.

@article{Reisner1980AllogeneicHS,
  title={Allogeneic hemopoietic stem cell transplantation using mouse spleen cells fractionated by lectins: in vitro study of cell fractions.},
  author={Yair Reisner and Susumu Ikehara and M Z Hodes and Robert A. Good},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1980},
  volume={77 2},
  pages={
          1164-8
        }
}
  • Y. Reisner, S. Ikehara, R. Good
  • Published 1 February 1980
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
Mouse spleen cells sequentially agglutinated by soybean agglutinin (SBA) and peanut agglutinin (PNA) previously had been shown to be sufficiently depleted of graft-versus-host activity to allow reconstitution of lethally irradiated allogeneic recipient mice. We have now tested the extent of T-cell depletion in this cell fraction by various in vitro assays, including cytotoxicity testing with anti-Thy-1 antiserum, mitogenic response to the T-cell mitogens concanavalin A and phytohemagglutinin… 

Figures and Tables from this paper

Enrichment for CFU-C from murine and human bone marrow using soybean agglutinin.

TLDR
Experiments mixing the human marrow fractions separated by SBA reveal that true enrichment for CFU-C is achieved in the unagglutinated fraction, as opposed to a possible depletion of a suppressor cell population.

Lectin separation of nonlymphoid suppressor cells induced by total lymphoid irradiation

TLDR
The presence of at least two suppressive populations induced by TLI is suggested: radioresistant SBA+, PNA+, SBA+PNA+ or PNA+SBA+ cells, and (b) radiosensitive PNA− and SBA-PNA− cells.

Separation of lectin-binding cells using polystyrene culture devices with covalently immobilized soybean agglutinin.

TLDR
The polystyrene technology described here could reduce or eliminate many of the drawbacks of soluble SBA agglutination, making SBA cell separation a viable and convenient technique for clinical application.

The Use of Soybean Agglutinin (SBA) for Bone Marrow (BM) Purging and Hematopoietic Progenitor Cell Enrichment in Clinical Bone Marrow Transplantation.

TLDR
Soybean agglutinin (SBA) binds very effectively to certain tumor cells, including neuroblastoma, breast cancer, and Burkitt's lymphoma cells, and has multiple potential applications for clinical bone marrow, transplantation (BMT).

Interferon‐independent, lectin‐induced augmentation of murine natural killer cell activity

TLDR
It is found that certain human, mouse and rat cell lines, which had low to moderate susceptibility to spontaneous murine NK activity, were sensitive to lectin‐induced NK‐cell‐mediated cytotoxicity while others were completely resistant.

Lectin receptors as lymphocyte surface markers.

  • N. Sharon
  • Biology, Medicine
    Advances in immunology
  • 1983

A Cell Surface Antigen Expressed on Mouse Lung Macrophages

TLDR
This study tested the binding ability of various lectins onto macrophages from various mouse organs as one of the approaches to answer the question of whether or not the lung macrophage belong to a separate subset from peritoneal Macrophages.