Allogeneic hemopoietic stem cell transplantation using mouse spleen cells fractionated by lectins: in vitro study of cell fractions.

@article{Reisner1980AllogeneicHS,
  title={Allogeneic hemopoietic stem cell transplantation using mouse spleen cells fractionated by lectins: in vitro study of cell fractions.},
  author={Yair Reisner and Susumu Ikehara and M Z Hodes and Robert A. Good},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1980},
  volume={77 2},
  pages={
          1164-8
        }
}
  • Y. Reisner, S. Ikehara, +1 author R. Good
  • Published 1 February 1980
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
Mouse spleen cells sequentially agglutinated by soybean agglutinin (SBA) and peanut agglutinin (PNA) previously had been shown to be sufficiently depleted of graft-versus-host activity to allow reconstitution of lethally irradiated allogeneic recipient mice. We have now tested the extent of T-cell depletion in this cell fraction by various in vitro assays, including cytotoxicity testing with anti-Thy-1 antiserum, mitogenic response to the T-cell mitogens concanavalin A and phytohemagglutinin… Expand
ALLOGENEIC BONE MARROW TRANSPLANTATION USING STEM CELLS FRACTIONATED BY LECTINS: VI, IN VITRO ANALYSIS OF HUMAN AND MONKEY BONE MARROW CELLS FRACTIONATED BY SHEEP RED BLOOD CELLS AND SOYBEAN AGGLUTININ
TLDR
Results were obtained with monkey bone-marrow cells, suggesting that monkeys can be used for evaluation of this fractionation technique for bone- marrow transplantation across histocompatibility barriers. Expand
Enrichment for CFU-C from murine and human bone marrow using soybean agglutinin.
TLDR
Experiments mixing the human marrow fractions separated by SBA reveal that true enrichment for CFU-C is achieved in the unagglutinated fraction, as opposed to a possible depletion of a suppressor cell population. Expand
Lectin separation of nonlymphoid suppressor cells induced by total lymphoid irradiation
TLDR
The presence of at least two suppressive populations induced by TLI is suggested: radioresistant SBA+, PNA+, SBA+PNA+ or PNA+SBA+ cells, and (b) radiosensitive PNA− and SBA-PNA− cells. Expand
Separation of lectin-binding cells using polystyrene culture devices with covalently immobilized soybean agglutinin.
TLDR
The polystyrene technology described here could reduce or eliminate many of the drawbacks of soluble SBA agglutination, making SBA cell separation a viable and convenient technique for clinical application. Expand
The Use of Soybean Agglutinin (SBA) for Bone Marrow (BM) Purging and Hematopoietic Progenitor Cell Enrichment in Clinical Bone Marrow Transplantation.
TLDR
Soybean agglutinin (SBA) binds very effectively to certain tumor cells, including neuroblastoma, breast cancer, and Burkitt's lymphoma cells, and has multiple potential applications for clinical bone marrow, transplantation (BMT). Expand
Interferon‐independent, lectin‐induced augmentation of murine natural killer cell activity
TLDR
It is found that certain human, mouse and rat cell lines, which had low to moderate susceptibility to spontaneous murine NK activity, were sensitive to lectin‐induced NK‐cell‐mediated cytotoxicity while others were completely resistant. Expand
The effect of peripheral lymphoid cells on the incidence of lethal graft versus host disease following allogeneic mouse bone marrow transplantation.
TLDR
Results raise the possibility that the fulminant GVHD seen in human marrow transplantation is in part due to the major contamination of bone marrow with peripheral blood that results from the techniques currently used for human bone marrow harvest. Expand
Lectin receptors as lymphocyte surface markers.
  • N. Sharon
  • Biology, Medicine
  • Advances in immunology
  • 1983
TLDR
The lectin receptors are characteristic markers of distinct lymphocyte subpopulations in mouse, man, and several other animals and are extremely useful for the identification and separation of lymphocytes, and therefore for studies on the functions and lineages of cells with a given phenotype. Expand
A Cell Surface Antigen Expressed on Mouse Lung Macrophages
TLDR
This study tested the binding ability of various lectins onto macrophages from various mouse organs as one of the approaches to answer the question of whether or not the lung macrophage belong to a separate subset from peritoneal Macrophages. Expand
Use of Lectins for Separation of Cells
TLDR
This chapter discusses the use of lectins for separation of cells, and peanut agglutinin (PNA) is the most popular one, being used for a variety of purposes in numerous laboratories, chiefly with murine and human lymphocytes but also with lymphocytes of other species. Expand
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