Allergenic and blastogenic reactivity of three antigens from Mycobacterium tuberculosis in sensitized guinea pigs.

@article{Worsaae1987AllergenicAB,
  title={Allergenic and blastogenic reactivity of three antigens from Mycobacterium tuberculosis in sensitized guinea pigs.},
  author={A Worsaae and Lars Ljungqvist and Kaare Robert Hasl\ov and I. D. Heron and J\orgen Bennedsen},
  journal={Infection and immunity},
  year={1987},
  volume={55 12},
  pages={2922-7}
}
Three antigens from a culture filtrate of Mycobacterium tuberculosis H37Rv were purified by affinity chromatography, using monoclonal antibodies. The molecular weights of the purified antigens are 17,000 to 19,000, 32,000 to 33,000, and 39,000, respectively, and by their migration patterns in sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing and nonreducing conditions, they all appeared to be single-chain polypeptides. Western blot and enzyme-linked immunosorbent assay… CONTINUE READING

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