Allantoate transport in Saccharomyces cerevisiae

  title={Allantoate transport in Saccharomyces cerevisiae},
  author={Vanessa Turoscy and Terrance G. Cooper},
  journal={Journal of Bacteriology},
  pages={971 - 979}
Allantoate uptake appears to be mediated by an energy-dependent active transport system with an apparent Michaelis constant of about 50 microM. Cells were able to accumulate allantoate to greater than 3,000 times the extracellular concentration. The rate of accumulation was maximum at pH 5.7 to 5.8. The energy source for allantoate uptake is probably different from that for uptake of the other allantoin pathway intermediates. The latter systems are inhibited by arsenate, fluoride, dinitrophenol… 
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Data suggest that citrate and succinate influx is mediated by a common plasma membrane transporter, not typical of fungi, as well as typical substrate specificity and mechanisms of functional activity of the dicarboxylate transporter in plasma membrane of S. cerevisiae.
Regulation of allantoate transport in wild-type and mutant strains of Saccharomyces cerevisiae
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Isolation and characterization of mutants that produce the allantoin-degrading enzymes constitutively in Saccharomyces cerevisiae
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Ureidosuccinate is transported by the allantoate transport system in Saccharomyces cerevisiae
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The response of GABA uptake to growth on various nitrogen sources suggested that uptake may be subject to several types of regulation and raised the possibility that a major portion of intracellular GABA is sequestered in the vacuole.
An overview of membrane transport proteins in Saccharomyces cerevisiae
All eukaryotic cells contain a wide variety of proteins embedded in the plasma and internal membranes, which ensure transmembrane solute transport. It is now established that a large proportion of
The allantoinase (DAL1) gene of Saccharomyces cerevisiae
Observations further support the roles of UASNTR and DAL7 UIS in the regulation of allantoin pathway gene expression.


Allantoin transport in Saccharomyces cerevisiae
Allantoin uptake in both growing and resting cultures of Saccharomyces cerevisiae occurs by a low-Km transport system that uses energy that is likely generated in the cytoplasm, based on the observation that transport did not occur in the absence of glucose or the presence of dinitrophenol, carbonyl cyanide-m-chloro-phenyl hydrazine, fluoride, or arsenate ions.
Ureidosuccinic acid permeation in Saccharomyces cerevisiae.
Metabolite compartmentation in Saccharomyces cerevisiae
Uninduced cultures of Saccharomyces cerevisiae exhibit high basal levels of allantoinase, allantoicase, and ureidoglycolate hydrolase, the enzymes responsible for degrading allantoIn to urea, which suggests that at times metabolite compartmentation may play an equal role with enzyme induction in the regulation ofallantoin metabolism.
Control of vacuole permeability and protein degradation by the cell cycle arrest signal in Saccharomyces cerevisiae
Data suggest that release of vacuolar constitutents and protein turnover may be regulated by the G1 arrest signal, and onset of nutrient starvation brought about release of large quantities of arginine and allantoin normally sequestered in the cell vacuole.
Urea transport in Saccharomyces cerevisiae
Urea transport in Saccharomyces cerevisiae occurs by two pathways and the first mode of uptake which occurs at external urea concentrations in excess of 0.5 mM is via either passive or facilitated diffusion.
Urea transport-defective strains of Saccharomyces cerevisiae
Experiments characterizing the urea active transport system in Saccharomyces cerevisiae indicate that formamide and acetamide are strong competitive inhibitors of urea accumulation, and adenosine 5'-triphosphate generated by glycolysis in conjunction with formation of an ion gradient is likely the driving force behind urea transport.
Regulation of histidine uptake by specific feedback inhibition of two histidine permeases in Saccharomyces cerevisiae.
It is concluded that the his-p1 mutant is histidine-permease-less, and that the activity of the histidine permeases are regulated by specific feedback inhibition.
Sequence of molecular events involved in induction of allophanate hydrolase
To elucidate the time intervals occupied by known processes involved in induction, temperature-sensitive mutants defective in messenger RNA transport from nucleus to cytoplasm (rna1) and in protein synthesis initiation (prt1) were employed along with an RNA polymerase inhibitor in experiments that measure cumulative synthetic capacity to produce allophanate hydrolase.
Requirement for HCO3- by ATP: urea amido-lyase in yeast.
Transport of -aminoisobutyric acid in Saccharomyces cerevisiae.