Allantoate amidinohydrolase (Allantoicase) from Chlamydomonas reinhardtii: its purification and catalytic and molecular characterization.

@article{Piedras2000AllantoateA,
  title={Allantoate amidinohydrolase (Allantoicase) from Chlamydomonas reinhardtii: its purification and catalytic and molecular characterization.},
  author={Pedro Piedras and Alfonso Mu{\~n}oz and Miguel Aguilar and Manuel Pineda},
  journal={Archives of biochemistry and biophysics},
  year={2000},
  volume={378 2},
  pages={
          340-8
        }
}
An allantoate-degrading enzyme has been purified to electrophoretic homogeneity for the first time from a photosynthetic organism, the unicellular green algae Chlamydomonas reinhardtii. The purification procedure included a differential protein extraction followed by conventional steps such as ammonium sulfate fractionation, gel filtration, anion exchange chromatography, and preparative electrophoresis. Under the routine assay conditions (7 mM allantoate), specific activity for the purified… 
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References

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Evolution of allantoinase and allantoicase involved in urate degradation in liver peroxisomes. A rapid purification of amphibian allantoinase and allantoicase complex, its subunit locations of the two enzymes, and its comparison with fish allantoinase and allantoicase.
TLDR
The results suggest that the evolution of fish to amphibian resulted in the dissociation of allantoicase into subunits and in the association ofallantoinase with allantoICase, and the two enzymes are lost by further evolution.
Purification and characterization of allantoate amidohydrolase from Bacillus fastidiosus.
TLDR
Allantoate amidohydrolase from Bacillus fastidiosus was purified 170-fold to homogeneity as judged by isoelectric focusing and nondenaturing and sodium dodecyl sulfate polyacrylamide gel electrophoresis, indicating the involvement of histidine or tyrosine residues in catalytic action.
Assay, purification and properties of allantoicase from Candida utilis.
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TLDR
The increased formation of L-ornithine, a constituent amino acid of gramicidin S, by arginase may be involved in the accelerated production of Gramicid in S by B. brevis in the presence ofL-arginine in the growth medium.
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TLDR
The enzyme did not hydrolyze allantoin and allantoic acid, which are intermediates in purine degradation, N-carbamoylsarcosine and citrulline, suggesting that it is a novel N- carbamoylamide amidohydrolase.
Ureidoglycollate lyase, a new metalloenzyme of peroxisomal urate degradation in marine fish liver.
TLDR
UGL was found to be essential in peroxisomal urate degradation, since allantoate, the intermediate of urate catabolism, was finds to be degraded to urea and glyoxylate in a two-step reaction catalysed by allantoicase, and UGL via S(-)-ureidoglycollate as an intermediate in fish liver peroxISomes, but not in a one- step reaction as previously believed.
Purification of Allantoinase from Soybean Seeds and Production and Characterization of Anti-Allantoinase Antibodies
TLDR
Allantoinase catalyzes the hydrolysis of allantoin to allantoic acid, a reaction important in both biogenesis and degradation of ureides, and was purified approximately 2500-fold from a crude extract of soybean seeds.
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