All-trans retinoic-acid inhibits heterodimeric bone morphogenetic protein 2/7-stimulated osteoclastogenesis, and resorption activity

  title={All-trans retinoic-acid inhibits heterodimeric bone morphogenetic protein 2/7-stimulated osteoclastogenesis, and resorption activity},
  author={Wen-juan Bi and Yi Liu and Jing Guo and Zhen Lin and Jinsong Liu and Miao Zhou and Daniel Wismeijer and Janak Lal Pathak and Gang Wu},
  journal={Cell \& Bioscience},
BackgroundBone regenerative heterodimeric bone morphogenetic protein 2/7 (BMP2/7) enhances but all-trans retinoic acid (ATRA) inhibits osteoclastogenesis. However, the effect of ATRA on physiological and/or BMP2/7-induced osteoclastogenesis in still unclear. In this study, we aimed to test the effect of combined treatment of BMP2/7 and ATRA on osteoclastogenesis, and resorption activity.ResultsAll-trans retinoic acid (1 µM) ± BMP2/7 (5 or 50 ng/ml) was added in murine pre-osteoclasts cell line… 
Retinoic acid increases the effect of bone morphogenetic protein type 2 on osteogenic differentiation of human adipose-derived stem cells
RA increased the effect of BMP-2 on osteogenic differentiation of human ASCs and promoted the highest BMP signaling pathway activation at days 7 and 14, and demonstrated more activation of differentiation of bone-forming cells than OM alone.
All‐trans retinoic acid and human salivary histatin‐1 promote the spreading and osteogenic activities of pre‐osteoblasts in vitro
It is found that co‐administration of Hst1 with ATRA additively stimulated the spreading and osteogenicity of pre‐osteoblasts on bio‐inert glass surfaces in vitro.
Human salivary histatin‐1 (Hst1) promotes bone morphogenetic protein 2 (BMP2)‐induced osteogenesis and angiogenesis
The results suggest that coadministration of Hst1 may enhance BMP2‐induced osteogenesis and angiogenesis, and thus may have potential for development into a treatment for large‐volume bone defects.
MiR‐137 knockdown promotes the osteogenic differentiation of human adipose‐derived stem cells via the LSD1/BMP2/SMAD4 signaling network
It is indicated that miR‐137 negatively regulated the osteogenic differentiation of hASCs via the LSD1/BMP2/SMAD4 signaling network, revealing a new potential therapeutic target of hASC‐based bone tissue engineering.
Implication of vinegar alone or in combination with Caltrate in a rat osteoporosis model induced by a low calcium diet and retinoic acid
1Jiangsu Hengshun Vinegar Industry Co., Ltd.,66 Hengshun Avenue, Dantu District, Zhenjiang 212143, China, 2School of Grain Science and Technology, Jiangsu University of Science and Technology,


BMP2/7 heterodimer can modulate all cellular events of the in vitro RANKL-mediated osteoclastogenesis, respectively, in different dose patterns.
Low-concentration BMP2/7 heterodimer may favor a rapid and spontaneous remodeling of its induced bone and, thus, bear a promising potential in cytokine-based tissue engineering.
Heterodimeric BMP-2/7 Antagonizes the Inhibition of All-Trans Retinoic Acid and Promotes the Osteoblastogenesis
Heterodimeric bone morphogenetic protein-2/7 bears a promising application potential to significantly promote bone regeneration and implant osteointegration for the patients with hypervitaminosis A and alcoholism.
Antagonistic and synergistic effects of bone morphogenetic protein 2/7 and all‐trans retinoic acid on the osteogenic differentiation of rat bone marrow stromal cells
It is shown that heterodimeric bone morphogenetic protein 2/7 (BMP2/7) could induce bone regeneration in a significantly higher dose‐efficiency in comparison with homodimerics BMPs.
Retinoic Acid Increases Proliferation of Human Osteoclast Progenitors and Inhibits RANKL-Stimulated Osteoclast Differentiation by Suppressing RANK
It is suggested that retinoic acid increases proliferation of human osteoclast progenitors and that it inhibits RANK-stimulated osteOClast differentiation by suppressing RANK.
Bone morphogenetic protein and retinoic acid signaling cooperate to induce osteoblast differentiation of preadipocytes
Results indicate that 3T3-F442A preadipocytes can be converted into fully differentiated osteoblasts in response to extracellular signaling cues, and BMP and retinoic acid signaling cooperate to stimulate cell proliferation, repress adipogenesis, and promote osteoblast differentiation.
Osteogenic differentiation of mouse adipose-derived adult stromal cells requires retinoic acid and bone morphogenetic protein receptor type IB signaling
It is reported here that significant osteogenesis, as determined by gene expression and histological analysis, is induced only when mouse ADAS are cultured in the presence of retinoic acid with or without recombinant human bone morphogenetic protein (BMP)-2 supplementation.
Retinoic acid inhibits NFATc1 expression and osteoclast differentiation
It is suggested that RA blocks in vitro RANKL-mediated osteoclastogenesis by decreasing NFATc1 function.
Retinoids inhibit differentiation of hematopoetic osteoclast progenitors
  • H. Conaway, E. Persson, +5 authors U. Lerner
  • Biology, Medicine
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 2009
Results suggest that activation of RARα by retinoids in myeloid hematopoietic precursor cells decreases osteoclast formation by altering expression of the transcription factors c‐Fos, NFAT2, and MafB.
Retinoids Stimulate Periosteal Bone Resorption by Enhancing the Protein RANKL, a Response Inhibited by Monomeric Glucocorticoid Receptor*
The data suggest that ATRA enhances periosteal bone resorption by increasing the RANKL/OPG ratio via RARα receptors, a response that can be inhibited by monomeric GR.
All-trans retinoic acid shifts rosiglitazone-induced adipogenic differentiation to osteogenic differentiation in mouse embryonic fibroblasts
Findings demonstrate that RSG in combination with ATRA promotes the commitment of MEFs to the osteoblast lineage, and the combination of these two agents may prove to be a promising and novel therapeutic regimen for insulin resistance without skeletal toxicity.