Alkylation of sulfhydryl groups on Galpha(s/olf) subunits by N-ethylmaleimide: regulation by guanine nucleotides.

@article{Giusti2003AlkylationOS,
  title={Alkylation of sulfhydryl groups on Galpha(s/olf) subunits by N-ethylmaleimide: regulation by guanine nucleotides.},
  author={Laura Giusti and Simone Taddei and Francesca Ceccarelli and Silvio Chericoni and Gianni Bigini and Antonio Lucacchini and Maria Rosa Mazzoni},
  journal={Biochimica et biophysica acta},
  year={2003},
  volume={1613 1-2},
  pages={7-14}
}
In rat striatum A(2A) adenosine receptors activate adenylyl cyclase through coupling to G(s)-like proteins, mainly G(olf) that is expressed at high levels in this brain region. In this study we report that the sulfhydryl alkylating reagent, N-ethylmaleimide (NEM), causes a concentration- and time-dependent inhibition of [3H] 2-p-(2-carboxyethyl)phenylethylamino)-5'-N-ethylcarboxamido adenosine ([3H]CGS21680) binding to rat striatal membranes. Membrane treatment with [14C]N-ethylmaleimide ([14C… CONTINUE READING