Alkylation of protein by methyl methanesulfonate and 1-methyl-1-nitrosourea in vitro.

Abstract

Methylation of horse heart cytochrome c has been examined in vitro with [methyl-14C]methanesulfonate (MMS) and [1-methyl-14C]-1-nitrosourea (MNU) as alkylating agents. Analysis of protein hydrolyzates by an automatic amino acid analyzer indicates that, at pH 9.0 with MMS, epsilon-N-monomethyl-lysine is found to be the only major methylated basic amino acid… (More)

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@article{Paik1984AlkylationOP, title={Alkylation of protein by methyl methanesulfonate and 1-methyl-1-nitrosourea in vitro.}, author={Woon Ki Paik and Peter DiMaria and S Kim and Peter N. Magee and Prabhakar D. Lotlikar}, journal={Cancer letters}, year={1984}, volume={23 1}, pages={9-17} }