Alkylation of individual genes in rat liver by the carcinogen N-nitrosodimethylamine.

@article{Milligan1988AlkylationOI,
  title={Alkylation of individual genes in rat liver by the carcinogen N-nitrosodimethylamine.},
  author={Justine Milligan and Michael C. Archer},
  journal={Biochemical and biophysical research communications},
  year={1988},
  volume={155 1},
  pages={14-7}
}
A method for quantifying alkylated bases in defined gene sequences of rat liver following carcinogen treatment is described. After restriction, the genomic DNA is heated to 65 degrees at pH 8.0 to cause depurination of 7-alkylguanine and 3-alkyladenine residues. Reaction with spermidine then generates strand breaks. After gel electrophoresis and Southern… CONTINUE READING