Alkaline phosphatase isoenzymes of liver and bone origin are incompletely resolved by wheat-germ-lectin affinity chromatography.

@article{Gonchoroff1989AlkalinePI,
  title={Alkaline phosphatase isoenzymes of liver and bone origin are incompletely resolved by wheat-germ-lectin affinity chromatography.},
  author={Daryl G. Gonchoroff and Earl L. Branum and J F O'brien},
  journal={Clinical chemistry},
  year={1989},
  volume={35 1},
  pages={29-32}
}
We used wheat-germ-lectin affinity chromatography as a tool to investigate the structure of alkaline phosphatase (ALP, EC 3.1.3.1) and to obtain fractions enriched in either bone or liver ALP activity. Liver and bone isoenzymes in serum samples were incompletely resolved except that the activity in the nonretained fraction (fraction 1) always represented pure liver isoenzyme and constituted a larger percentage of total activity in pooled sera with increased liver ALP activity than in pooled… CONTINUE READING

Similar Papers

Loading similar papers…