Alkaline phosphatase is an almost perfect enzyme.

  title={Alkaline phosphatase is an almost perfect enzyme.},
  author={T T Simopoulos and William P. Jencks},
  volume={33 34},
The second-order rate constant, kcat/km, for catalysis of the hydrolysis of 4-nitrophenyl phosphate by alkaline phosphatase decreases with increasing viscosity in the presence of sucrose or arabinose, with a slope of delta[kcat/Km)0/(kcat/Km)]/delta(eta/eta 0) = 1.4 at pH 8.0, 25 degrees C. This is consistent with rate-limiting diffusional encounter of the… CONTINUE READING