Alkaline phosphatase induces the mineralization of sheets of collagen implanted subcutaneously in the rat.

@article{Beertsen1992AlkalinePI,
  title={Alkaline phosphatase induces the mineralization of sheets of collagen implanted subcutaneously in the rat.},
  author={Wouter Beertsen and Theo van den Bos},
  journal={The Journal of clinical investigation},
  year={1992},
  volume={89 6},
  pages={1974-80}
}
To determine whether alkaline phosphatase (ALP) can cause the mineralization of collagenous matrices in vivo, bovine intestinal ALP was covalently bound to slices of guanidine-extracted demineralized bovine dentin (DDS). The preparations were implanted subcutaneously over the right half of the rat skull. Control slices not treated with the enzyme were implanted over the left half of the skull of the same animals. Specimens were harvested after periods varying from 1 to 4 wk. It was shown that… CONTINUE READING