Alkaline conditions in hydrophilic interaction liquid chromatography for intracellular metabolite quantification using tandem mass spectrometry.

@article{Teleki2015AlkalineCI,
  title={Alkaline conditions in hydrophilic interaction liquid chromatography for intracellular metabolite quantification using tandem mass spectrometry.},
  author={Attila Teleki and Andr{\'e}s S{\'a}nchez-Kopper and Ralf Takors},
  journal={Analytical biochemistry},
  year={2015},
  volume={475},
  pages={4-13}
}
Modeling of metabolic networks as part of systems metabolic engineering requires reliable quantitative experimental data of intracellular concentrations. The hydrophilic interaction liquid chromatography-electrospray ionization-tandem mass spectrometry (HILIC-ESI-MS/MS) method was used for quantitative profiling of more than 50 hydrophilic key metabolites of cellular metabolism. Without prior derivatization, sugar phosphates, organic acids, nucleotides, and amino acids were measured under… CONTINUE READING

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