Alkaline Gel Electrophoresis Assay to Detect DNA Strand Breaks and Repair Mechanisms in Escherichia coli

@inproceedings{Mattos2009AlkalineGE,
  title={Alkaline Gel Electrophoresis Assay to Detect DNA Strand Breaks and Repair Mechanisms in Escherichia coli},
  author={Jos{\'e} Carlos Pelielo de Mattos and Ellen Serri da Motta and M{\'a}rcia Bet{\^a}nia Nunes de Oliveira and Fl{\'a}vio Jos{\'e} da Silva Dantas and Adriano Caldeira de Araujo},
  year={2009}
}
Reactive oxygen species (ROS) can induce lesions in different cellular targets, including DNA. Stannou s chloride (SnCl 2) is a ROS generator, leading to lethality in Esche richia coli (E. coli), with the base excision repai r (BER) mechanism playing a role in this process. Many tech niques have been developed to detect genotoxicity, as comet assay, in… CONTINUE READING