[Alkaline DNAse from rat brain].

Abstract

A DNAse was isolated from rat brain and purified 1100-fold using affinity chromatography on a column with DNA-agarose and chromatography on granulated hydroxyapatite. The electrophoretically pure Mg2+, Mn2+-dependent enzyme preparation hydrolyzes the denaturated DNA with a maximal activity at pH 8,4. The optimal terminal concentration of Mg2+ corresponds to the Mg/phosphorus molar ratio of the substrate is 1:2. For Mn2+ this correlation is 1:1. Using the immobilized substrate method, the exonuclease type of DNAse activity has been established. The enzyme activity depends on the state of its SH-groups; the reaction is inhibited by pCMB. The molecular weight of DNAase as determined by gel-filtration through Sephadex G-200 is equal to 60 000.

Cite this paper

@article{Ivanov1980AlkalineDF, title={[Alkaline DNAse from rat brain].}, author={Vasil A. Ivanov and T M Tret'iak and Azhub I. Gaziev and B F Santalov}, journal={Biokhimii︠a︡}, year={1980}, volume={45 5}, pages={912-22} }