Alignment of conduits for the nascent polypeptide chain in the ribosome-Sec61 complex.

@article{Beckmann1997AlignmentOC,
  title={Alignment of conduits for the nascent polypeptide chain in the ribosome-Sec61 complex.},
  author={Roland Beckmann and Doryen Bubeck and Robert A. Grassucci and Pawel Penczek and Adriana Verschoor and Gunter Blobel and Joachim Frank},
  journal={Science},
  year={1997},
  volume={278 5346},
  pages={2123-6}
}
An oligomer of the Sec61 trimeric complex is thought to form the protein-conducting channel for protein transport across the endoplasmic reticulum. A purified yeast Sec61 complex bound to monomeric yeast ribosomes as an oligomer in a saturable fashion. Cryo-electron microscopy of the ribosome-Sec61 complex and a three-dimensional reconstruction showed that the Sec61 oligomer is attached to the large ribosomal subunit by a single connection. Moreover, a funnel-shaped pore in the Sec61 oligomer… CONTINUE READING