Alignment-Free Design of Highly Discriminatory Diagnostic Primer Sets for Escherichia coli O104:H4 Outbreak Strains

@inproceedings{Pritchard2012AlignmentFreeDO,
  title={Alignment-Free Design of Highly Discriminatory Diagnostic Primer Sets for Escherichia coli O104:H4 Outbreak Strains},
  author={Leighton Pritchard and Nicola J Holden and Martina Bielaszewska and Helge Karch and Ian K. Toth},
  booktitle={PloS one},
  year={2012}
}
BACKGROUND An Escherichia coli O104:H4 outbreak in Germany in summer 2011 caused 53 deaths, over 4000 individual infections across Europe, and considerable economic, social and political impact. This outbreak was the first in a position to exploit rapid, benchtop high-throughput sequencing (HTS) technologies and crowdsourced data analysis early in its investigation, establishing a new paradigm for rapid response to disease threats. We describe a novel strategy for design of diagnostic PCR… CONTINUE READING
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