Alemtuzumab induces caspase-independent cell death in human chronic lymphocytic leukemia cells through a lipid raft-dependent mechanism

@article{Mone2006AlemtuzumabIC,
  title={Alemtuzumab induces caspase-independent cell death in human chronic lymphocytic leukemia cells through a lipid raft-dependent mechanism},
  author={Andrew P. Mone and Carolyn M. Cheney and Amy L. Banks and Susheela Tridandapani and Najma S Mehter and Sara K Guster and T Lin and Charles F. Eisenbeis and Donn C. Young and John C. Byrd},
  journal={Leukemia},
  year={2006},
  volume={20},
  pages={272-279}
}
Alemtuzumab is a humanized IgG1 kappa antibody directed against CD52, a glycosyl-phosphatidylinositol linked cell-membrane protein of unknown function. Herein, we demonstrate that alemtuzumab promotes rapid death of chronic lymphocytic leukemia (CLL) cells in vitro, in a complement and accessory cell free system. Using minimal detergent solubilization of CLL membranes, we found that CD52 colocalizes with ganglioside GM-1, a marker of membrane rafts. Fluorescence microscopy revealed that upon… CONTINUE READING

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