Aldosterone regulation of sodium channel gamma-subunit mRNA in cortical collecting duct cells.

@article{Denault1996AldosteroneRO,
  title={Aldosterone regulation of sodium channel gamma-subunit mRNA in cortical collecting duct cells.},
  author={Deanna Denault and G{\'e}za Fejes-T{\'o}th and Anik{\'o} N{\'a}ray-Fejes-T{\'o}th},
  journal={The American journal of physiology},
  year={1996},
  volume={271 1 Pt 1},
  pages={C423-8}
}
Specific regulatory mechanisms of aldosterone-stimulated Na+ reabsorption through the apical amiloride-sensitive channel are unknown. In this study, we examined the effects of aldosterone on Na+ channel gamma-subunit mRNA levels in cultured rabbit cortical collecting duct cells. With the use of reverse transcriptase-polymerase chain reaction (RT-PCR) with RNA isolated from aldosterone-treated cells and degenerate primers, a 446-base pair (bp) PCR product was amplified and further characterized… CONTINUE READING