Aldosterone alters the phospholipid composition of rat colonocytes

  title={Aldosterone alters the phospholipid composition of rat colonocytes},
  author={Libor Mrnka and Olga Novakova and Franti{\vs}ek Nov{\'a}k and Eva Tvrzicka and Jiř{\'i} P{\'a}cha},
  journal={The Journal of Steroid Biochemistry and Molecular Biology},
Previous studies have shown that aldosterone treatment of amphibian epithelial cells results not only in stimulation of Na(+) absorption but also in changes in phospholipid composition which are necessary for the mineralocorticoid action of aldosterone. The present study was designed to investigate the effect of aldosterone on phospholipids of mammalian epithelia. Phospholipid and fatty acid composition was examined in colonic epithelium (mineralocorticoid target tissue) and thymus (non… Expand
Corticosteroid effect on Caco-2 cell lipids depends on cell differentiation
Aldosterone had a profound influence on lipid metabolism in enterocytes and that its effect depends on the stage of differentiation, which may reflect a physiologically important phenomenon with long-term consequences for membrane structure and function. Expand
Endogenously Expressed Epithelial Sodium Channel Is Present in Lipid Rafts in A6 Cells*
It is demonstrated that a fraction of α, β, and γ ENaC is present in detergent-insoluble membranes, that subunits exist in membranes that float on discontinuous sucrose density gradients, and that methyl-β-cyclodextrin treatment causes a redistribution of EN aC subunits to higher density membranes. Expand
Intracellular pH regulation in colonocytes of rat proximal colon.
Proximal colonocytes are able to maintain a more stable pH(i) than distal cells, which seems to be facilitated by their higher intrinsic buffer capacity, which is likely involved in the regulation of pH( i) in vivo. Expand
Corticosteroid regulation of colonic ion transport during postnatal development: methods for corticosteroid analysis.
The purpose of the present review is to summarize the mechanism of sodium and potassium transport pathways and show how these transport processes change postnatally and how hormones, particularly corticosteroids, modify the pattern of development. Expand
ENaC–Membrane Interactions
These findings demonstrate a novel regulatory mechanism of ENaC, which could be blocked by cooling and trapping these agents in the rigidified membrane, consistent with effects on the membrane-dielectric properties. Expand
Investigating lipid headgroup composition within epithelial membranes: a systematic review.
A comprehensive systematic literature search to ascertain the key lipid components of epithelial membranes is carried out, with a particular focus on addressing the human GIT and to use compositional data to understand structural aspects of biological membranes. Expand
Molecular approach of gossypol-induced reproductive toxicity in male rabbits. Changes in seminal plasma amino acids and fatty acids.
Evaluating the effects of two sublethal doses of gossypol on some amino and fatty acid concentrations in male rabbit seminal plasma showed that total amino acids, total essential amino acids (EAA), total non-essential amino acids(EAA) and EAA/non-EAA ratio were decreased in a dose-dependent manner during gOSSypol treatment. Expand


The effect of aldosterone on phospholipid and phosphoinositide metabolism in rat submandibular gland.
The purpose of this study was to determine if aldosterone stimulates membrane phospholipid or phosphoinositide turnover in the rat salivary gland and duct and to report on the turnover of membrane phosphate fractions or percentage fractions. Expand
Mineralocorticoids modify rat liver delta 6 desaturase activity and other parameters of lipid metabolism.
The changes in linoleyl-CoA desaturase activity of rat liver microsomes were studied after a single intraperitoneal injection of 11-deoxycorticosterone or aldosterone at physiological doses. FattyExpand
Variations in dietary triacylglycerol saturation alter the lipid composition and fluidity of rat intestinal plasma membranes.
The results indicate that the lipid composition, fluidity and enzyme activities of intestinal plasma membranes can be altered by dietary means and that rat enterocytes possess regulatory mechanisms which modulate the cholesterol content of the microvillus membranes so as to mitigate changes in lipid fluidity. Expand
Dexamethasone-induced alterations in lipid composition and fluidity of rat proximal-small-intestinal brush-border membranes.
Results demonstrate that dexamethasone administration can modulate the lipid fluidity and lipid composition of rat proximal-small-intestinal brush-border membranes. Expand
Dietary triacylglycerol modulates sodium-dependent D-glucose transport, fluidity and fatty acid composition of rat small intestinal brush-border membrane.
Comparison of the kinetic parameters of Na+-dependent D-glucose transport revealed that fish oil-membrane vesicles had a higher maximum velocity (Vmax) than butter fat membrane vesicle vesicular velocity but a similar Km for glucose. Expand
Effects of an acetyl-coenzyme A carboxylase inhibitor and a sodium-sparing diuretic on aldosterone-stimulated sodium transport, lipid synthesis, and phospholipid fatty acid composition in the toad urinary bladder.
The results support the conclusion that aldosterone increases Na+ transport in the toad urinary bladder by altering membrane fatty acid metabolism and that the lipid biosynthetic events following ald testosterone treatment are a primary response to the hormone and not secondary to increased Na+ Transport. Expand
Estrogen modulates ileal basolateral membrane lipid dynamics and Na+-K+-ATPase activity.
Ethinyl estradiol effects on basolateral membrane lipid dynamics are confined to the ileum and are associated with inhibition of Na+-K+-ATPase activity, related to specific modifications in the availability of phospholipid after estrogen treatment. Expand
Aldosterone-stimulated transmethylations are linked to sodium transport.
It is concluded that Aldo stimulates PL methylation, protein carboxymethylation, and turnover of PC from choline, and inhibition of methylation reactions coincides with the inhibition of ISC response to Aldo. Expand
Phospholipids and Electrolyte Transport
  • T. Yorio, L. W. Frazier
  • Chemistry, Medicine
  • Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine
  • 1990
A number of observations point to a major role of lipid second messengers that activate enzymes involved in protein phosphorylations, i.e., protein kinase C, as major regulators of a variety of ion channels and transporters. Expand
Phospholipid Changes during Adaptation to Acidosis in Urinary Bladder of Bufo marinus 1
  • L. W. Frazier, T. Yorio
  • Biology, Medicine
  • Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine
  • 1989
Whether phospholipids play a role in the adaptation to metabolic acidosis by toad urinary bladder epithelium is investigated and a higher rate of arachidonic acid incorporation into several PL in acidotic animals is revealed. Expand