Alcoholysis and reverse hydrolysis reactions in organic one-phase system with a hyperthermophilic beta-glycosidase.

@article{GarcaGaribay2000AlcoholysisAR,
  title={Alcoholysis and reverse hydrolysis reactions in organic one-phase system with a hyperthermophilic beta-glycosidase.},
  author={Mariano Garc{\'i}a-Garibay and Agust{\'i}n L{\'o}pez-Mungu{\'i}a and Eduardo B{\'a}rzana},
  journal={Biotechnology and bioengineering},
  year={2000},
  volume={69 6},
  pages={627-32}
}
Alcoholysis and reverse hydrolysis reactions were performed enzymatically in one-phase water-saturated 1-heptanol systems. Lactose or glucose was used as substrate to produce heptyl-beta-galactoside and/or heptyl-beta-glucoside, respectively. When alcoholysis of lactose was performed at 37 degrees C with beta-galactosidase from Escherichia coli, the initial rate was 14 nmol/mL min, and the limiting factors were the poor solubility of the substrate in 1-heptanol and low thermal stability of the… CONTINUE READING