Alcohol dehydrogenases from olive (Olea europaea) fruit.

  title={Alcohol dehydrogenases from olive (Olea europaea) fruit.},
  author={Joaqu{\'i}n J. Salas and Jacinto S{\'a}nchez},
  volume={48 1},
Alcohol dehydrogenase activity was detected in extracts from the pericarp tissues of developing olive fruits using hexanal as the substrate. Total activity in the crude extract was 20-fold higher with NADPH than with NADH. Three discrete enzymes were resolved by means of a purification protocol involving ammonium sulfate fractionation followed by ion-exchange and affinity chromatography. One of the enzymes was NAD-dependent and displayed a high K(m) for hexanal (K(m) = 2.1 mM). Two NADP… CONTINUE READING
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