Corpus ID: 6227537

Alanosine toxicity in Novikoff rat hepatoma cells due to inhibition of the conversion of inosine monophosphate to adenosine monophosphate.

@article{Graff1976AlanosineTI,
  title={Alanosine toxicity in Novikoff rat hepatoma cells due to inhibition of the conversion of inosine monophosphate to adenosine monophosphate.},
  author={J. Graff and P. Plagemann},
  journal={Cancer research},
  year={1976},
  volume={36 4},
  pages={
          1428-40
        }
}
2-Amino-3-(hydroxynitrosoamino)propionic acid (alanosine), at a concentration as low as 2.7 muM, completely inhibits the incorporation of hypoxanthine into adenosine triphosphate by cultured Novikoff rat hepatoma cells. Alanosine inhibits the first step in the conversion of inosine monophosphate to adenosine monophosphate because inosine monophosphate, but not adenylosuccinate, accumulates in treated cells. However, the alanosine inhibition is not prevented by aspartic acid, even at a… Expand
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A novel synergistic effect of alanosine and guanine on adenine nucleotide synthesis in mammalian cells. Alanosine as a useful probe for investigating purine nucleotide metabolism
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  • Biology, Medicine
  • Journal of cellular physiology
  • 1980
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Results suggest that guanine nucleotides exert a regulatory effect on the activity of the enzyme adenylosuccinate synthetase, and indicates that alanosine provides a useful probe for investigating the regulation of purine nucleotide metabolism in mammalian cells. Expand
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References

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THYMIDINE TRANSPORT BY CULTURED NOVIKOFF HEPATOMA CELLS AND UPTAKE BY SIMPLE DIFFUSION AND RELATIONSHIP TO INCORPORATION INTO DEOXYRIBONUCLEIC ACID
TLDR
Results from pulse-chase experiments indicate that a rapid turnover of dTTP to thymidine may be involved in limiting the level of thymine nucleotides in the cell, and indicates that uridine andThymidine are transported by different systems. Expand
NUCLEOTIDE POOLS IN NOVIKOFF RAT HEPATOMA CELLS GROWING IN SUSPENSION CULTURE
  • P. Plagemann
  • Biology, Medicine
  • The Journal of cell biology
  • 1972
TLDR
Evidence is presented to indicate that it is the cytoplasmic pool that expands during incubation with high concentrations of nucleosides in the medium, whereas the nuclear pool remains constant and very small in size. Expand
Purine and pyrimidine transport by cultured Novikoff cells. Specificities and mechanism of transport and relationship to phosphoribosylation.
TLDR
Evidence indicates that specific transport processes precede the phosphoribosyltransferase reactions and are the rate-limiting step in purine incorporation by whole cells. Expand
Mode of action of alanosine.
TLDR
Evidence indicates that alanosine may interfere with the conversion of inosine monophosphate to adenosine monphosphate, and inhibition of RNA adenine synthesis is not antagonized by glutamine or aspartate. Expand
Nucleotide pools of Novikoff rat hepatoma cells growing in suspension culture. I. Kinetics of incorporation of nucleosides into nucleotide pools and pool sizes during growth cycle
Results from kinetic studies on the incorporation of 3H‐5‐uridine and 3H‐8‐adenosine into the acid‐soluble nucleotide poor and nucleic acids by Novikoff hepatoma cells (subline N1S1‐67) in suspensionExpand
Nucleotide pools of Novikoff rat hepatoma cells growing in suspension culture. II. Independent snucleotide pools for nucleic acid synthesis
TLDR
Evidence is presented which suggests that one uridine nucleotide pool is located in the cytoplasm and another in the nucleus and that mainly the nuclear pool supplies nucleotides for RNA synthesis. Expand
Mengovirus Replication in Novikoff Rat Hepatoma and Mouse L Cells: Effects on Synthesis of Host-Cell Macromolecules and Virus-specific Synthesis of Ribonucleic Acid
TLDR
Novikoff cells (strain N1S1-67) and L-67 cells, a nutritional mutant of the common strain of mouse L cells, were infected with mengovirus under identical experimental conditions, and there was a secondary increase in activity in both types of cells coincidental with virus-induced disintegration of the host cells. Expand
Fluctuations of DNA‐dependent RNA polymerase and synthesis of macromolecules during the growth cycle of Novikoff rat hepatoma cells in suspension culture
TLDR
The transition of suspension cultures of Novikoff rat hepatoma cells from the exponential to the stationary phase is accompanied by decreases of over 90% in the rates of synthesis of RNA, DNA and protein, and a disaggregation of the polyribosomes with a concomitant accumulation of 80 S and 110 S ribosomal structures. Expand
Replication of mengovirus. I. Effect on synthesis of macromolecules by host cell.
TLDR
The replication of mengovirus was studied in two strains of Novikoff (rat) hepatoma cells propagated in vitro and revealed a marked depression of ribonucleic acid (RNA) and protein synthesis by strain N1S1-63 and Mengovirus had no effect on either protein or RNA synthesis or on the DNA-dependent RNA polymerase activity of a second strain. Expand
Detection of an unstable RNA in chick fibroblasts after reduction of the UTP pool by glucosamine.
TLDR
Analysis of the distribution of radioactivity among the acidsoluble intermediates revealed a general applicability of the method to study the RNA metabolism and to establish corresponding precursorships. Expand
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