Alanine scanning mutagenesis of insulin.

  title={Alanine scanning mutagenesis of insulin.},
  author={Claus Kristensen and Thomas L. Kjeldsen and Finn Christoph Wiberg and Lauge Sch{\"a}ffer and M Hach and Svend Havelund and Joseph Bass and Donald F. Steiner and Asser Sloth Andersen},
  journal={The Journal of biological chemistry},
  volume={272 20},
Alanine scanning mutagenesis has been used to identify specific side chains of insulin which strongly influence binding to the insulin receptor. A total of 21 new insulin analog constructs were made, and in addition 7 high pressure liquid chromatography-purified analogs were tested, covering alanine substitutions in positions B1, B2, B3, B4, B8, B9, B10, B11, B12, B13, B16, B17, B18, B20, B21, B22, B26, A4, A8, A9, A12, A13, A14, A15, A16, A17, A19, and A21. Binding data on the analogs revealed… CONTINUE READING
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