Alanine scanning mutagenesis of a plant virus movement protein identifies three functional domains.

@article{GiesmanCookmeyer1993AlanineSM,
  title={Alanine scanning mutagenesis of a plant virus movement protein identifies three functional domains.},
  author={D. Giesman-Cookmeyer and Steven A. Lommel},
  journal={The Plant cell},
  year={1993},
  volume={5 8},
  pages={973-82}
}
Alanine scanning mutagenesis was performed on the red clover necrotic mosaic virus (RCNMV) movement protein (MP), and 12 mutants were assayed in vitro for RNA binding characteristics and in vivo for their ability to potentiate RCNMV cell-to-cell movement. The mutant phenotypes that were identified in vitro and in vivo suggest both that cooperative RNA binding is not necessary for cell-to-cell movement in vivo and that only a fraction of the wild-type RNA binding may be required. The MP mutants… CONTINUE READING

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