Alamethicin as a permeabilizing agent for measurements of Ca(2+)-dependent ATPase activity in proteoliposomes, sealed membrane vesicles, and whole cells.

@article{Ritov1993AlamethicinAA,
  title={Alamethicin as a permeabilizing agent for measurements of Ca(2+)-dependent ATPase activity in proteoliposomes, sealed membrane vesicles, and whole cells.},
  author={V. Ritov and M. K. Murzakhmetova and I. Tverdislova and E. Menshikova and A. A. Butylin and Avakian TYu and L. Yakovenko},
  journal={Biochimica et biophysica acta},
  year={1993},
  volume={1148 2},
  pages={
          257-62
        }
}
The channel-forming antibiotic peptide alamethicin was used in measurements of Ca-ATPase activity in sarcoplasmic reticulum (SR) vesicles, proteoliposomes containing Ca(2+)-ATPase from SR, and native human platelets. Alamethicin was used as a permeabilizing agent providing for a free access of the whole cells or sealed vesicles interiors for ions, ATP, and other reactants. The experiments were carried out with the use of alamethicin preparations obtained in our laboratory and that purchased… Expand
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