• Corpus ID: 90802203

Aislamiento y caracterización de una posible metiltransferasa dam proveniente del bacteriófago no lambdoide mep021

@inproceedings{Cuevas2014AislamientoYC,
  title={Aislamiento y caracterizaci{\'o}n de una posible metiltransferasa dam proveniente del bacteri{\'o}fago no lambdoide mep021},
  author={A. Cuevas and Edith Milena},
  year={2014}
}
In this work was determined the frame of open lecture 5, of bacteriophague mEp021 not lambdoide, which show a significant identity (E=5e-14, E=7e-11 y E=4e-10 by regular blast) with the sequences methyltransferases of phagues. Therefore it was extracted the DNA of phagues mEp021. The primers was designed from its corresponding sequence then simplified by PCR the methyltransferase gen with am awaited size of 348 pb, it was cloned in a transitory vector TOPO TA. The identification of positive… 

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TLDR
In BALB/c mice inoculated orally or intravenously with the dam mutant, the median lethal dose was at least 10(6)-fold higher than the MLD of the wild-type.
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TLDR
This work has identified a new nonlambdoid phage family and identified another immunity group with 48 members that were not induced by UV light treatment, and no recombinants were obtained when crossed with either lambda or lambdaBLK20.
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The bacteriophage T4 dam gene, encoding the Dam DNA [N6-adenine]methyltransferase (MTase), has been subcloned into the plasmid expression vector, pJW2. In this construct, designated pINT4dam,
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TLDR
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TLDR
The T2 dam and T4 dam promoters are not identical and appear to have slightly different efficiencies; when fused to the E. coli lacZ gene, the T4 promoter produces about twofold more beta-galactosidase activity than does the T2 promoter.
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TLDR
The authors cloned the dam gene of Yersinia enterocolitica and showed that Dam is essential for viability, and overproduction of Escherichia coli-derived Dam results in increased tissue culture invasion of Y. enterocol itica, implying different roles or activities of Dam in mismatch repair of the two species.
The GATATC‐modification enzyme EcoRV is closely related to the GATC‐recognizing methyltransferases DpnII and dam from E. coli and phage T4
The amino acid sequence of EcoRV DNA methyltransferase which methylates the amino group of the 5′‐adenine residue of the target sequence GATATC has been found to be closely related to that of three
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TLDR
It is proposed that the E. coli Dam methyl enzyme may be directly involved in the process of methylation-instructed mismatch repair and that the T4 Dam methylase is unable to substitute for the E coli enzyme.
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TLDR
The inviability of double dam, rec mutations became more comprehensible with the observation that dam mutations result in enhanced expression of genes of the SOS regulon, implying that a dam mutation results in a requirement for higher levels of these proteins.
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TLDR
Insertional inactivation of lic2A, but not lgtC or lgtF, leads to resistance to phage infection and to the same structure of the LOS as observed among phase-variable phage-resistant variants, indicating that in the H. influenzae Rd LOS only the first two sugars extending from the third heptose are part of bacterial phage receptors.
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