Airborne environmental DNA for terrestrial vertebrate community monitoring

  title={Airborne environmental DNA for terrestrial vertebrate community monitoring},
  author={Christina Lynggaard and Mads Frost Bertelsen and Casper V Jensen and Matthew Stanley Johnson and Tobias Guldberg Fr{\o}slev and Morten Tange Olsen and Kristine Bohmann},
  journal={Current Biology},
  pages={701 - 707.e5}

Measuring biodiversity from DNA in the air

Spatio-temporal variability of eDNA signal and its implication for fish monitoring in lakes

Environmental DNA (eDNA) metabarcoding is revolutionizing the monitoring of aquatic biodiversity. The use of eDNA has the potential to enable non-invasive, cost-effective, time-efficient and

Environmental DNA Metabarcoding: A Novel Contrivance for Documenting Terrestrial Biodiversity

Simple Summary The innovative concept of environmental DNA has found its foot in aquatic ecosystems but remains an unexplored area of research concerning terrestrial ecosystems. When making

A review of applications of environmental DNA for reptile conservation and management

Abstract Reptile populations are in decline globally, with total reptile abundance halving in the past half century, and approximately a fifth of species currently threatened with extinction.

Quantitative environmental DNA metabarcoding reflects quantitative capture data of fish community obtained by electrical shocker

Quantitative assessment of biodiversity provides invaluable information for ecosystem conservation and the sustainable use of biological resources. Environmental DNA (eDNA) metabarcoding has been

Short range contributions of local sources to ambient air

Recent developments in aerobiology have enabled the investigation of airborne biomass with high temporal and taxonomic resolution. In this study, we assess the contributions of local sources to

Optimised DNA isolation from marine sponges for natural sampler DNA (nsDNA) metabarcoding

It is found that homogenisation may not be required for sponge tissue and more starting material does not necessarily improve taxon detection, which combined provide an optimised DNA isolation procedure for sponges to enhance marine biodiversity assessment using natural sampler DNA metabarcoding.

Toward quantitative metabarcoding

By modeling the process through which amplicon-sequence data arises, rather than transforming the data post-hoc, it is shown how to estimate the starting DNA proportions from a mixture of many taxa and opens the door to improve the use of metabarcoding data in a wide range of applications in ecology, public health, and related fields.

Strategies for sample labelling and library preparation in DNA metabarcoding studies

An overview of the three main workflows for sample‐specific labelling and library preparation in metabarcoding studies on Illumina sequencing platforms; one‐step PCR, two‐ step PCR, and tagged PCR is presented.

Existing and emerging uses of drones in restoration ecology

In the absence of effective and scalable human intervention, up to 95% of the world's ecosystems will be affected by anthropogenic degradation by 2050. Therefore, immediate and large‐scale ecological



Measuring biodiversity from DNA in the air

Airborne environmental DNA metabarcoding for the monitoring of terrestrial insects - a proof of concept

It is suggested that airborne eDNA has the potential to become a powerful tool for terrestrial biodiversity monitoring, with many impactful applications including the monitoring of pests, invasive or endangered species or disease vectors.

Environmental DNA for wildlife biology and biodiversity monitoring.

Species detection using environmental DNA from water samples

A novel approach, based on the limited persistence of DNA in the environment, to detect the presence of a species in fresh water, using specific primers that amplify short mitochondrial DNA sequences to track the existence of a frog in controlled environments and natural wetlands.

Investigating the Potential Use of Environmental DNA (eDNA) for Genetic Monitoring of Marine Mammals

To determine the potential use of eDNA for genetic monitoring, specific primers that amplify short mitochondrial DNA sequences were used to detect the presence of a marine mammal, the harbor porpoise, in a controlled environment and in natural marine locations.

Environmental DNA enables detection of terrestrial mammals from forest pond water

The results presented here show that the eDNA metabarcoding approach is also promising even in forest mammal biodiversity surveys, with results similar to those of aquatic/semiaquatic systems.

Debugging diversity – a pan‐continental exploration of the potential of terrestrial blood‐feeding leeches as a vertebrate monitoring tool

It is found that vertebrate blood ingested by haematophagous terrestrial leeches throughout their distribution is a viable source of DNA with which to examine a wide range of vertebrates.

Environmental DNA (eDNA) metabarcoding of pond water as a tool to survey conservation and management priority mammals

Environmental DNA (eDNA) metabarcoding can identify terrestrial taxa utilising aquatic habitats alongside aquatic communities, but terrestrial species’ eDNA dynamics are understudied. We evaluated

Environmental DNA metabarcoding as a useful tool for evaluating terrestrial mammal diversity in tropical forests.

  • J. L. MenaH. Yagui A. Lyet
  • Environmental Science
    Ecological applications : a publication of the Ecological Society of America
  • 2021
This study is among the first to show the large potential of eDNA metabarcoding for assessing Amazonian mammal communities, providing, in combination with conventional techniques, a rapid overview of mammal diversity with broad applications to monitoring, management and conservation.