Air-liquid interface (ALI) culture of human bronchial epithelial cell monolayers as an in vitro model for airway drug transport studies.

@article{Lin2007AirliquidI,
  title={Air-liquid interface (ALI) culture of human bronchial epithelial cell monolayers as an in vitro model for airway drug transport studies.},
  author={Hongxia Lin and Hong Li and Hyun-Jong Cho and Shengjie Bian and Hwan-Jung Roh and Min-ki Lee and Jung Sun Kim and Suk-Jae Chung and Chang-koo Shim and Dae-Duk Kim},
  journal={Journal of pharmaceutical sciences},
  year={2007},
  volume={96 2},
  pages={341-50}
}
Serially passaged normal human bronchial epithelial (NHBE) cell monolayers were established on Transwell inserts via an air-liquid interface (ALI) culture method. NHBE cells were seeded on polyester Transwell inserts, followed by an ALI culture from day 3, which resulted in peak TEER value of 766+/-154 Omegaxcm2 on the 8th day. Morphological characteristics were observed by light microscopy and SEM, while the formation of tight junctions was visualized by actin staining, and confirmed… CONTINUE READING

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