Agroinfection as a rapid method for propagating Cowpea mosaic virus-based constructs.

  title={Agroinfection as a rapid method for propagating Cowpea mosaic virus-based constructs.},
  author={Li Liu and G. P. Lomonossoff},
  journal={Journal of virological methods},
  volume={105 2},
To increase the efficiency of infections with Cowpea mosaic virus (CPMV)-based constructs, clones suitable for agroinfection were constructed. Full-length copies of RNA-1 and RNA-2 were inserted between the sequence of a Cauliflower mosaic virus (CaMV) 35S promoter and a nos terminator and were introduced into the Agrobacterium tumefaciens plasmid, pBINPLUS. Infiltration of leaves of either Nicotiana benthamiana or cowpea (Vigna unguiculata) with a bacterial suspension containing a mixture of… CONTINUE READING


Publications citing this paper.
Showing 1-10 of 24 extracted citations

OBPC Symposium: Maize 2004 & beyond—Plant virus-based vectors in agriculture and biotechnology

In Vitro Cellular & Developmental Biology - Plant • 2007
View 4 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…