Corpus ID: 87303285

Agrobacterium tumefaciens mediated transgenic tetraploid Isatis indigotica carrying anti-insect gene from Pinella Ternata Agglutinin

@article{Ruxian2003AgrobacteriumTM,
  title={Agrobacterium tumefaciens mediated transgenic tetraploid Isatis indigotica carrying anti-insect gene from Pinella Ternata Agglutinin},
  author={Ding Ru-xian},
  journal={Chinese Traditional and Herbal Drugs},
  year={2003}
}
  • Ding Ru-xian
  • Published 2003
  • Biology
  • Chinese Traditional and Herbal Drugs
Object Pinella Ternata Agglutinin (PTA) gene was conducted into tetraploid Isatis indigotica Fort. to get anti-pest species.Methods Agrobacterium tumefaciens mediated transformation in which the “high toxic” strain EHA105 and the plant binary expression vector pCAMB I A2200 were adopted under CaMV 35S promotor and neomycin phosphorus transferred enzyme (NptⅡ) served as the selection marker.Results The plant regeneration rate of transgenic explants was up to 95% and transgenic frequency was 10… Expand
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