Agrobacterium tumefaciens-mediated transformation of the dermatophyte, Trichophyton mentagrophytes: an efficient tool for gene transfer.

@article{Yamada2009AgrobacteriumTT,
  title={Agrobacterium tumefaciens-mediated transformation of the dermatophyte, Trichophyton mentagrophytes: an efficient tool for gene transfer.},
  author={Tsuyoshi Yamada and K. Makimura and Kazuo Satoh and Yoshiko Umeda and Yumiko Ishihara and Shigeru Abe},
  journal={Medical mycology},
  year={2009},
  volume={47 5},
  pages={485-94}
}
Agrobacterium tumefaciens-mediated transformation (ATMT) was used to facilitate gene transfer into the clinically important dermatophyte, Trichophyton mentagrophytes (teleomorph: Arthroderma vanbreuseghemii). A binary vector containing a hygromycin B resistance cassette was introduced into A. tumefaciens, and the resultant strain was co-cultivated with fungal small conidia. Transformation yielded a large number of hygromycin B-resistant transformants. Hybridization analysis showed that most of… CONTINUE READING