Agrobacterium tumefaciens-mediated transformation ofMucor circinelloides

  title={Agrobacterium tumefaciens-mediated transformation ofMucor circinelloides},
  author={Ildik{\'o} Nyilasi and Kl{\'a}ra {\'A}cs and Tibor Norbert Papp and E´va Nagy and Cs V{\'a}gv{\"o}lgyi},
  journal={Folia Microbiologica},
TheAgrobacterium tumefaciens-mediated transformation of the zygomycetous fungusMucor circinelloides is described. A method was also developed for the hygromycin B-based selection ofMucor transformants. Transformation with the hygromycin B phosphotransferase gene ofEscherichia coli controlled by the heterologousAspergillus nidulans trpC promoter resulted in hygromycin B-resistant clones. The presence of the hygromycin resistance gene in the genome of the transformants was verified by polymerase… CONTINUE READING
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