Agrobacterium tumefaciens-mediated transformation of Pinus pinea L. cotyledons: an assessment of factors influencing the efficiency of uidA gene transfer

@article{Humara1999AgrobacteriumTT,
  title={Agrobacterium tumefaciens-mediated transformation of Pinus pinea L. cotyledons: an assessment of factors influencing the efficiency of uidA gene transfer},
  author={Jaime M. Humara and Mari{\'a}n L{\'o}pez and Ricardo Javier Ord{\'a}s},
  journal={Plant Cell Reports},
  year={1999},
  volume={19},
  pages={51-58}
}
 This study is the first report of a protocol for transfer and expression of foreign chimeric genes into cotyledons excised from Pinus pinea L. embryos. Agrobacterium tumefaciens EHA105 harbouring the plasmid p35SGUSint was more infective than LBA4404 or C58 GV3850, as determined by the percentage of cotyledons showing uidA expression. Factors which significantly affected the T-DNA transfer included: (1) preinduction and concentration of bacteria, (2) days of coculture and (3) the wounding… CONTINUE READING
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